Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a , b TEM images of P-CD@MM. c Measurements of the expression levels of CD86 and CD206 in each group. d Representative fluorescence images of cellular uptake of P-CD@MM in 4T1 or NIH-3T3. e , f Relative cell viability of NIH-3T3 and 4T1 cells after different treatments ( n = 6 independent samples). g , h Live/dead cell and ROS staining of 4T1 cells after different treatments. For ( a – d , g , h ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via two-tailed Student’s t test. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Expressing, Fluorescence, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a Confocal images of CRT exposure in 4T1 cells after different treatments. b , c Evaluation of HMGB1 and ATP levels in 4T1 cells after different treatments ( n = 6 independent samples). d A scheme to show the experimental process of the evaluation of DC maturation (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). e , f Evaluation of DC maturation level in each group ( n = 6 mice). g – i Flow cytometry analysis and the corresponding quantitative results of CD86 + and CD206 + expression in TAMs of BMDM after different treatments ( n = 6 mice). For ( a ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Flow Cytometry, Expressing

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a CRT staining of tumor tissues after different treatments ( n = 3 mice). b Expression levels of CD4 + T cells and CD8 + T cells in the primary tumors after different treatments ( n = 6 mice). c Expression levels of CD86 + and CD206 + in TAMs in the primary tumors after different treatments ( n = 6 mice). d Schematic diagram of P-CD@MM-mediated PDT to induce an immune response in vivo (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). For ( a ), experiment was repeated three times independently with similar results.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Staining, Expressing, In Vivo

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting FAM83D triggers tumor cell senescence via cGAS-STING signaling activation and reprograms TAMs to combat glioma

doi: 10.1186/s13046-026-03681-y

Figure Lengend Snippet: Knockdown of FAM83D leads to the infiltration of M1 TAMs. A Expression of genes associated with chemotaxis, IL1 production, IL6 production, and IFN-γ production in RNA-seq of the U251 cell line. B UMAP plot of TAMs from scRNA-seq colored by sub-clusters. C Expression of M1 markers ( Cd86 , Ccl3 , and Tnf ) and M2 markers ( Mrc1 , Arg1 , and Tgfbi ) in TAMs from scRNA-seq. D GO biological process enrichment based on the corresponding marker genes in each TAMs sub-cluster. p adjusted value, a corrected p value determined by bonferroni. E Proportion of each TAM’s sub-cluster across samples, respectively. p value, Chi-square test. F Enrichment scores of M1/M2 macrophage associated signatures in TAMs across samples. p value, two-sided unpaired Wilcoxon test. G Spatial structure model of glioma based on spatial transcriptomics. H Representative sample UKF266 showing the expression of FAM83D in each layer (left) and quantification of FAM83D positive spots in each layer (right). p value, two-sided unpaired t-test; error bars, mean ± SEM. I Representative samples UKF269 and UKF275 showing the relationship between the expression of FAM83D and the proportion of inflammatory macrophage (left) and their correlation analysis (right). p value, Pearson’s correlation test. J Representative HE staining image and staining image of Iba1, iNOS, and Cd206 in mouse brains under different treatments (top) and quantification of protein level normalized to the control group (bottom). Each data point represents one mouse ( n = 3 mice for each treatment). p value, two-sided unpaired t-test; error bars, mean ± SEM. K Schematic of cellular experiments verifying the effect of SASP induced by knockdown of FAM83D on macrophage polarization. L Relative expression of M1 markers ( Tnf , Il1b , and Cd86 ) and M2 markers ( Tgfb, Il10 , and Cd206 ) in the BV2 cell line treated with different conditioned medium. Each data point represents one biological replicate ( n = 3 replicates for each treatment). The culture time is 48 h. p value, two-sided unpaired t-test; error bars, mean ± SEM. M Representative staining image of Cd86 and Cd206 in the BV2 cell line co-cultured with tumor cells under different conditions (left) and quantification of protein level normalized to the control group (right). Each data point represents one random region of interest ( n = 10 random regions for each condition). The culture time is 48 h. p value, two-sided unpaired t-test; error bars, mean ± SEM

Article Snippet: Cells were incubated with rabbit anti-FAM83D antibody (Abcam, no. ab236882, 1:100), mouse anti-Ki67 antibody (Cell Signaling Technology, no. 9449 S, 1:1000), rabbit anti-γH2AX antibody (Cell Signaling Technology, no. 9718 S, 1:500), mouse anti-α-Tubulin antibody (Cell Signaling Technology, no. 3873 S, 1:1000), mouse anti-dsDNA antibody (Santa Cruz Biotechnology, no. sc-58749, 1:50), rabbit anti-STING antibody (Proteintech, no. 19851-1-AP, 1:100), rabbit anti-Cd206 antibody (Proteintech, no. 18704-1-AP, 1:500) or rabbit anti-Cd86 antibody (Abclonal, no. A1199, 1:100) overnight at 4 °C.

Techniques: Knockdown, Expressing, Chemotaxis Assay, RNA Sequencing, Marker, Spatial Transcriptomics, Staining, Control, Cell Culture